This course offers comprehensive training in reproductive biotechnologies applied to equines, integrating both clinical and laboratory approaches. Techniques such as follicular aspiration, intracytoplasmic sperm injection, in vitro embryo production, and embryo transfer are analyzed in depth, highlighting the factors that influence their technical and reproductive efficiency. Key aspects for the planning, equipment, and environmental control of specialized laboratories are also addressed. Finally, emerging technologies such as in vitro gamete production, embryo sexing, advanced cryopreservation, and gene editing are explored, offering a critical look at the future of assisted reproduction in equines.